Normally our eyes consists of a organ called retina where the photoreceptor cells are present in it. When the light enters into our eyes, it will reach the photoreceptors and the photoreceptors makes us possible to see. However, the lights doesn't hit the photoreceptors remains undetected. Now, nocturnal animals like owl and cats need to see better at night. Hence, their eyes consists of another layer below the retina called tapetum lucidum. Now, when the light doesn't hit the photoreceptors, it reaches the tapetum lucidum. This layer acts like a mirror. It reflects light back to the photoreceptors. Thus, giving them a second chance to detect light. Some of this reflected light also comes out of the animal's eyes. Thus, making it seem that their eyes are glowing.
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